週次 |
日期 |
單元主題 |
第1週 |
2/16 |
Introduction
Specific staining for cell organelles |
第2週 |
2/23 |
Antibody production, purification |
第3週 |
3/02 |
Gel electrophoresis and Western blotting |
第4週 |
3/09 |
Gel electrophoresis and Western blotting
(Lab) |
第5週 |
3/16 |
Primary tissue culture technique |
第6週 |
3/23 |
Culture of cell lines |
第7週 |
3/30 |
Purification of DNA and mRNA, Northern and Southern blotting |
第8週 |
4/06 |
PCR & RT-PCR: theory, primer design strategy
Real time RT-PCR: theory |
第9週 |
4/13 |
Localization of mRNA by in situ hybridization, Cell death assays |
第10週 |
4/20 |
Transfection
siRNA |
第11週 |
4/27 |
Microarray : theory and evaluation
Flow Cytometry (cell sorting) |
第12週 |
5/04 |
Isolation of mitochondria |
第13週 |
5/11 |
Proteomics: theory and application |
第14週 |
5/18 |
EMSA (electrophoretic mobility shift assay) |
第15週 |
5/25 |
Radioimmunoassay |
第16週 |
6/01 |
Introducing macromolecules into cells |
第17週 |
6/08 |
Final Examination |